Fig. 4

Transcription dynamics in growing oocytes. a Barchart showing time of first detection of genes in growing oocytes, according to classification as reference gene, from canonical TSS (w/o upTSS) or novel upstream TSS (upTSS), or novel multi- or monoexonic gene. The total numbers of genes classified as expressed in each RNA-seq datasets are given in Additional file 6: Table S5. b Browser screenshots of representative early-methylating (Igf2r, Zac1) and late-methylating (Cdh15, Nnat) gDMRs in relation to RNA-seq data from different stages of the oocyte growth and DNA methylation acquired in 60–65 µm oocytes. In the RNA annotation track, red gene structures are transcribed from left to right and blue gene structures from right to left, with arrows showing the most upstream TSSs and direction of transcription. RNA-seq data show that transcriptional pattern is established prior to DNA methylation establishment