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Fig. 4 | Epigenetics & Chromatin

Fig. 4

From: The quest for epigenetic regulation underlying unisexual flower development in Cucumis melo

Fig. 4

Carpel and Stamen epigenetic analysis of melon transcription factors. a H3K9ac and H3K27me3 profiles and RNA levels of four transcription factors in female and male flowers. Two TFs specific of the male flowers (MELO3C003778 and MELO3C0100515) and two specific of the female flowers (MELO3C002691 and MELO3C022209) were selected for epigenetic profiling in isolated carpels and stamens of melon flowers. The distributions of peaks and RNA reads obtained in our genome-wide analyses are plotted above the genome model for each TF. b Stamens and carpels of melon flowers were dissected by Laser Microdissection (LMD) and ChIP-qPCR assays were performed with anti-H3K9ac and anti-H3K27me3 antibodies. The upper panels show an example of dissections performed with the LCM technique. The bar charts represent the qPCR assays on immune-precipitated DNA using primers annealing a neighboring sequence to the TSS as indicated in panel A (purple bar)

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