Fig. 2
From: Stabilization of Foxp3 expression by CRISPR-dCas9-based epigenome editing in mouse primary T cells
dCas9-TET1CD-mediated demethylation of the Foxp3 CNS2 locus. a Sequence at the Foxp3 CNS2 locus is shown. Each gRNA sequence is underlined and numbered #C2-1 to #C2-10. Specific CpG sites are lettered red. b and c The methylation status of CpG sites at the Foxp3 CNS2 locus in dCas9-TET1CD and each gRNA-expressing 68-41 cells (b) and dCas9-TET1CD or the TET1CD catalytic mutant and gRNA #C2-7 expressing 68-41 cells (c) was determined by bisulfite sequence analysis. The 68-41 cells stably expressing dCas9-TET1CD were transduced with each gRNA expression lentivirus and sorted (b). The 68-41 cells were co-transduced with dCas9-TET1CD or TET1CD mutant and gRNA CNS2#C2-7 and sorted (c). A horizontal row depicts one sequenced clone in which CpGs was methylated (black) or demethylated (white). Data are pooled from two independent experiments