Fig. 1
From: Stabilization of Foxp3 expression by CRISPR-dCas9-based epigenome editing in mouse primary T cells
CRISPR-dCas9-based epigenome editing for primary T cells. a A retroviral vector for the expression of dCas9-epigenome regulator fusion proteins from Moloney murine leukemia virus promoter long terminal repeats (ΔLTRs) and green fluorescent protein (GFP) from an internal ribosomal entry site (IRES). Retroviral and lentiviral vector for bicistronic expression of the gRNA from a U6 promoter (U6) and DsRed from a short EF1a promoter (EFS). b Protein expression of dCas9-epigenome regulator fusion proteins in transfected HEK293T cells was detected by western blot against anti-Flag antibody. Anti α-tubulin antibody was used for loading control