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Table 1 Purity of GFP-positive fractions obtained at sampling or after organ culture

From: Fetal testis organ culture reproduces the dynamics of epigenetic reprogramming in rat gonocytes

Age of sampling Percentage of GFP-positive cells Percentage of gonocytes based on morphology
16.5 dpc 92.85 ± 1.13% (n = 13) 96.26 ± 1.09% (n = 13)
18.5 dpc 91.88 ± 1.18% (n = 4) 97.34 ± 1.55% (n = 4)
20.5 dpc 78.33 ± 2.91% (n = 4) 99.27 ± 0.73% (n = 4)
3 dpp 91.59 ± 2.78% (n = 5) 97.06 ± 2.24% (n = 5)
16.5 dpc + 2 d 66.00 ± 4.88% (n = 5) 97.71 ± 2.29% (n = 5)
16.5 dpc + 4 d 61.85 ± 9.04% (n = 4) 83.75 ± 13.89% (n = 4)
18.5 dpc + 3 d 61.27 ± 6.00% (n = 5) 97.01 ± 1.23% (n = 5)
  1. Purity was evaluated on a hemocytometer based on fluorescence and morphology. All data are expressed as mean ± SEM (n = 4–13)