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Table 1 Purity of GFP-positive fractions obtained at sampling or after organ culture

From: Fetal testis organ culture reproduces the dynamics of epigenetic reprogramming in rat gonocytes

Age of sampling

Percentage of GFP-positive cells

Percentage of gonocytes based on morphology

16.5 dpc

92.85 ± 1.13% (n = 13)

96.26 ± 1.09% (n = 13)

18.5 dpc

91.88 ± 1.18% (n = 4)

97.34 ± 1.55% (n = 4)

20.5 dpc

78.33 ± 2.91% (n = 4)

99.27 ± 0.73% (n = 4)

3 dpp

91.59 ± 2.78% (n = 5)

97.06 ± 2.24% (n = 5)

16.5 dpc + 2 d

66.00 ± 4.88% (n = 5)

97.71 ± 2.29% (n = 5)

16.5 dpc + 4 d

61.85 ± 9.04% (n = 4)

83.75 ± 13.89% (n = 4)

18.5 dpc + 3 d

61.27 ± 6.00% (n = 5)

97.01 ± 1.23% (n = 5)

  1. Purity was evaluated on a hemocytometer based on fluorescence and morphology. All data are expressed as mean ± SEM (n = 4–13)