Fig. 5

H19 and Snrpn DMR methylation profiles in gonocytes and somatic cells in vivo and in vitro. a 7 CpG and 5 CpG sites were analyzed in H19 (black circles) and Snrpn (white circles) DMR, respectively. The average percentage of methylation was obtained in GFP-positive gonocytes (b) and GFP-negative somatic cells (c) at different stages in vivo and in vitro. Data represent the average % methylation ± SEM (n = 3/time point). ^a,b p < 0.05 between different stages in vivo using a one-way ANOVA followed by a Tukey’s post hoc test. ^# p < 0.05, ^#### p ≤ 0.0001 between 18.5 dpc and after culture using a Student unpaired t test