Fig. 3

Global changes in H3K4me3 in gonocytes and Sertoli cells in vivo and in vitro. Co-staining of H3K4me3 (red) with HSP90 (green: marker of gonocyte) was done on sections of rat testes sampled in vivo (a) or after organ culture (b). Gonocytes (arrow head) and Sertoli cells (arrow) can be observed on sections of testis samples at different stages in vivo and in vitro. Immunofluorescence intensity was quantified in gonocytes (c) and in Sertoli cells (d). Data represent the average normalized fluorescence intensity ± SEM (n = 3/time point). ^a,b p < 0.05 between different stages in vivo using a one-way ANOVA followed by a Tukey’s post hoc test. *p < 0.05, **p ≤ 0.01, ***p ≤ 0.001 between 16.5 dpc and different times of culture using a one-way ANOVA followed by a Tukey’s post hoc test. Scale = 50 μm