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Table 3 Number of biological replicates analyzed per exposure

From: Differential DNA methylation at conserved non-genic elements and evidence for transgenerational inheritance following developmental exposure to mono(2-ethylhexyl) phthalate and 5-azacytidine in zebrafish

Samples per exposure group

Gen

Length

DNAa

RNAa

Global methylationb

RRBSb

BisPCR2b

3 dpf Larvae

F0

68–76

     

6 dpf Larvae

F0

50–60

6

5

6

5

6

15 dpf Larvae

F0

10

5

 

5

 

5

Brain per gender

F0

 

15

 

15

  

Liver per gender

F0

 

15

 

15

  

Sperm

F0

 

15

 

15

 

6

6 dpf Larvae

F1

30

9

 

9

 

5

15 dpf Larvae

F1

30

9

 

9

  

6 dpf Larvae

F2

30

9

 

9

 

5

  1. aPool of 10 or 2 larvae for 6 or 15 dpf, respectively
  2. bNumber of biological replicates used in specific methylation analysis
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Epigenetics & Chromatin

ISSN: 1756-8935