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Table 3 Number of biological replicates analyzed per exposure

From: Differential DNA methylation at conserved non-genic elements and evidence for transgenerational inheritance following developmental exposure to mono(2-ethylhexyl) phthalate and 5-azacytidine in zebrafish

Samples per exposure group Gen Length DNAa RNAa Global methylationb RRBSb BisPCR2b
3 dpf Larvae F0 68–76      
6 dpf Larvae F0 50–60 6 5 6 5 6
15 dpf Larvae F0 10 5   5   5
Brain per gender F0   15   15   
Liver per gender F0   15   15   
Sperm F0   15   15   6
6 dpf Larvae F1 30 9   9   5
15 dpf Larvae F1 30 9   9   
6 dpf Larvae F2 30 9   9   5
  1. aPool of 10 or 2 larvae for 6 or 15 dpf, respectively
  2. bNumber of biological replicates used in specific methylation analysis