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Fig. 6 | Epigenetics & Chromatin

Fig. 6

From: Differential DNA methylation at conserved non-genic elements and evidence for transgenerational inheritance following developmental exposure to mono(2-ethylhexyl) phthalate and 5-azacytidine in zebrafish

Fig. 6

Locus-specific methylation analysis of different larval stages and sperm over generation following exposure to mono(2-ethylhexyl) phthalate (MEHP) and 5-azacytidine (5AC). a Loci showing significant effects with both 10 and 25 µM 5AC exposures compared to control at F0 6 dpf. Error bars represent SEM. b Hierarchical clustering of generational effects on DNA methylation differences of MEHP and 5AC exposures at 6 and 15 dpf and in sperm from F0 compared to their respective generational or tissue-specific controls (ward clustering). c Heat map showing the methylation difference compared to control of all CpG sites over generations (F0, F1 and F2) and 15 dpf (15) and sperm (sp) with at least one significant differentially methylated CpG, exhibiting a methylation difference of more than 10% as indicated by the black squares. Each stage or tissue-specific sample was compared with the controls from the same stage

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