Fig. 7
From: Internal modifications in the CENP-A nucleosome modulate centromeric dynamics
CENP-A mutations in K124 display altered replication timing of centromeric foci. a EdU-pulsed cells containing CpA/K124A/K124Q were co-stained with CENP-B (CpB) to assess percentage of centromeric replicating origins at early, mid and late S phases. 5 μm scale bars are located in the bottom right of the merge panel. Boxed to the right is an example of automated co-localization analysis using ImageJ, which was used to determine the fraction of co-localizing CpB and EdU foci (in white) with EdU +/CpB + insets to show co-localization. b A graphical representation of the percentage of centromeric origins co-stained with EdU (CpB + EdU) over the total number of centromeric (marked by CpB) foci