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Fig. 4 | Epigenetics & Chromatin

Fig. 4

From: Internal modifications in the CENP-A nucleosome modulate centromeric dynamics

Fig. 4

K124A/Q display normal centromeric localization. a CpA/K124A/K124Q were fused to an N-terminal GFP tag and co-expressed with mCh-CENP-A (CpA). The co-localization finder plug-in in ImageJ was used to find co-localizing GFP- and mCh-foci to indicate centromeric deposition, and final panel depicts a complete merge of all three channels including DAPI. b CENP-C (CpC) immunofluorescence (IF) was performed on GFP-tagged CpA/K124A/K124Q to look for co-localization. Puncta are boxed and magnified (in white) in the co-localize channel

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