Fig. 3

FLII is functionally associated with MLL1/2 complexes and governs HOX gene expression. a Endogenous FLII was immunoprecipitated from HeLa cell extracts, and the immunoprecipitates were probed for the presence of RbBP5, menin, MLL1 or MLL2 as indicated. b Same as Fig. 2a except total RNA was prepared from control cells or cells individually depleted for FLII, RbBP5 and WDR5 (SET1/MLL component subunit) by siRNAs or co-depleted for FLII and the indicated SET1/MLL components. RNA was reverse transcribed and cDNA was used in RT-qPCR to monitor DLX3 gene expression. Data represent as the average of three independent experiments performed in triplicate ± SEM. c FLII was depleted from HeLa by two independent siRNAs, and RT-qPCR was performed to monitor the expression of HOXA9, HOXB3, MEIS1 and MEOX1 (n = 3, T test, *p < 0.05; **p < 0.01; ***p < 0.001, n.s. not significant)