Fig. 2

FLII controls DLX3 gene expression. a FLII was depleted from HeLa by two independent siRNAs. Total RNA was prepared from control cells or cells transfected with either SENP3 or FLII siRNA. RNA was reverse transcribed and cDNA was used in real-time quantitative PCR (RT-qPCR) to monitor the expression of DLX3 and HOXC8. Normalized values (against GAPDH mRNA level) represent the average of three independent experiments performed in triplicate ± SEM (n = 3, T test, ***p < 0.001, n.s. not significant). The lower panel demonstrates efficient depletion of SENP3 or FLII. b Same as in a except flag-FLII domains were expressed. Data represent the average values from three independent experiments in duplicate ± SEM. c Same as in a except co-depletion of FLII together with SENP3 was performed. The lower panel is a representative western blot, demonstrating efficient depletion of SENP3 and FLII