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Fig. 1 | Epigenetics & Chromatin

Fig. 1

From: Flightless-I governs cell fate by recruiting the SUMO isopeptidase SENP3 to distinct HOX genes

Fig. 1

FLII is a major novel interactor of SENP3. a Immunoprecipitation using anti-SENP3 antibody was performed on extracts from HeLa cells. Immunoprecipitates were analyzed by SDS-PAGE and probed with antibodies as indicated. b Similar to a except, SENP3 was depleted from HeLa cells by siRNA. After 72 h of siRNA transfection, SENP3 IP was performed. SDS-PAGE and immunoblotting with selected antibodies were performed as indicated. c Same as b but antibody directed against FLII was used for immunoprecipitation. d Schematic representation of FLII domain structure. The numbers in brackets indicate the amino acids. Equal amounts of in vitro transcribed/translated SENP3 were mixed with either GST only as a control or with GST-tagged FLII fragments (GST-LRR, GST-GelA and GST-GelB) and GST pull-down was performed. After intensive washing, bound proteins were eluted in SDS-sample buffer, separated by SDS-PAGE and probed for the presence of SENP3

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