Fig. 7From: Dynamics of 5-methylcytosine and 5-hydroxymethylcytosine during pronuclear development in equine zygotes produced by ICSI5-Hydroxymethylcytosine (5hmC) patterns in the maternal (mPN) and paternal (pPN) pronucleus during pronuclear development. 5hmC, in green, was clearly present throughout pronuclear development in both the mPN and the pPN. The parental origin of the pronuclei was determined by H3K9me3 immunostaining (in blue) and the DNA was stained by EthD-2 (in red). All the images were taken at 630×. The scale bar represents 20 μmBack to article page