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Fig. 6 | Epigenetics & Chromatin

Fig. 6

From: Dynamics of 5-methylcytosine and 5-hydroxymethylcytosine during pronuclear development in equine zygotes produced by ICSI

Fig. 6

Dynamics of global DNA methylation in the paternal (pPN) and the maternal (mPN) pronucleus. In the pPN, no differences were found either in the normalized 5mC (5mC/DNA) or in the total DNA fluorescence (DNA) between pronuclear stages. However, an increase in the total 5mC fluorescence (5mC) was observed between PN1 and PN2. In the mPN, an increase in the normalized 5mC (5mC/DNA) was found between PN1 and PN2. Similarly, an increase in the total 5mC fluorescence (5mC) was found between PN1 and PN2–PN3–PN4, and in the total DNA fluorescence (DNA) between PN1 and PN3. Finally, higher levels of normalized 5mC (5mC/DNA) were found in the mPN than the pPN at PN3 (*). The distribution of the 60 embryos included in the study was: PN1 (n = 6), PN2 (n = 25), PN3 (n = 18) and PN4 (n = 11). Different superscripts (a or b) indicate significant differences, and p values <0.05 were considered significant

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