Fig. 3

Total DNA fluorescence of the paternal (pPN) and maternal (mPN) pronucleus in each pronuclear stage. Comparisons were made between the different pronuclear stages independently for the pPN and the mPN; 141 zygotes were included in the study (PN1 n = 19, PN2 n = 59, PN3 n = 37 and PN4 n = 26). In the pPN, significant differences were observed between PN1 and PN3-PN4. In the mPN, significant differences were found between PN1 and PN2-PN3-PN4. The data were analyzed with SPSS Statistics 24, by the Kruskal–Wallis H test combined with Bonferroni correction for multiple testing. Different superscripts (a or b) indicate significant differences, and p values <0.05 were considered significant