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Fig. 5 | Epigenetics & Chromatin

Fig. 5

From: Silencing markers are retained on pericentric heterochromatin during murine primordial germ cell development

Fig. 5

HP1γ is recruited to pericentric heterochromatin in E10.5–E13.5 germ cells. a Nuclear spread preparations from E10.5 to E11.5 embryos and from E13.5 male and female gonads were stained for HP1γ (green). This protein is also stably recruited to pericentric heterochromatin of PGCs, in the three stages examined. E10.5 and E11.5 germ cells are marked with OCT4 (red), while E13.5 germ cells are marked with TRA98 (red). Four or more embryo trunks or gonads were pooled for the nuclear spread preparations and 20–30 PGC, and somatic nuclei were recorded. Representative germ cells are marked with yellow dashed circles. Scale bars represent 10 μm. b Quantification analysis of HP1γ levels on the whole nuclear area (N.A.) and pericentric heterochromatin regions (PHC) in the different embryonic stages examined. The levels of HP1γ isoform at E10.5 and E11.5 in the pericentric heterochromatin regions are notably more enriched compared to those of the corresponding somatic cells. Asterisks (* or **) indicate significant differences

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