Table 2 Antibody specifications for each demethylase IF assay
From: Assessing histone demethylase inhibitors in cells: lessons learned
Demethylase | Histone mark | Primary antibody for histone mark (cat. no.) | Dilution | Secondary antibody for histone mark (cat. no.) | Dilution | Primary antibody for FLAG-tag (cat. no.) | Dilution | Secondary antibody for FLAG-tag (cat. no.) | Dilution |
|---|---|---|---|---|---|---|---|---|---|
KDM3A | H3K9me2 | Abcam (ab1220) | 1:500 | Alexafluor 488 (MG2A20) | 1:500 | F7425 | 1:500 | A-11011 | 1:500 |
KDM4A/B/C | H3K9me3 | Abcam (ab8898-100) | 1:500 | Alexafluor 488 (A-11070) | 1:500 | F3165 | 1:500 | A21124 | 1:500 |
KDM6B | H3K27me3 | Millipore (07-449) | 1:500 | Alexafluor 488 (A-11070) | 1:500 | F3165 | 1:500 | A21124 | 1:500 |
KDM5A/B/C | H3K4me3 | Diagenode (C15410003-50) | 1:500 | Alexafluor 488 (A-11070) | 1:500 | F3165 | 1:500 | A21124 | 1:500 |