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Table 2 Antibody specifications for each demethylase IF assay

From: Assessing histone demethylase inhibitors in cells: lessons learned

Demethylase Histone mark Primary antibody for histone mark (cat. no.) Dilution Secondary antibody for histone mark (cat. no.) Dilution Primary antibody for FLAG-tag (cat. no.) Dilution Secondary antibody for FLAG-tag (cat. no.) Dilution
KDM3A H3K9me2 Abcam (ab1220) 1:500 Alexafluor 488 (MG2A20) 1:500 F7425 1:500 A-11011 1:500
KDM4A/B/C H3K9me3 Abcam (ab8898-100) 1:500 Alexafluor 488 (A-11070) 1:500 F3165 1:500 A21124 1:500
KDM6B H3K27me3 Millipore (07-449) 1:500 Alexafluor 488 (A-11070) 1:500 F3165 1:500 A21124 1:500
KDM5A/B/C H3K4me3 Diagenode (C15410003-50) 1:500 Alexafluor 488 (A-11070) 1:500 F3165 1:500 A21124 1:500