Fig. 6
From: Assessing histone demethylase inhibitors in cells: lessons learned
Cytotoxic activity of different KDM inhibitors in live cells. a High-content images of HeLa cells treated with 100 nM paclitaxel for 24 h and stained with Annexin V (green) and Yo-Pro (red). Apoptotic cells were defined as Annexin V positive with or without Yo-Pro 3 uptake; necrotic cells were defined as Yo-Pro 3 positive; and healthy cells were defined as Annexin V and Yo-Pro 3 negative, b percentage of healthy, apoptotic and necrotic HeLa cells treated with doxorubicin, paclitaxel or staurosporine in a dose-dependent manner, c percentage of healthy, apoptotic and necrotic HeLa cells treated with KDM inhibitors of the KDOAM series  or CPI-455 for 24 h in a dose-dependent manner, c immunofluorescence assay showing the effect of doxorubicin or paclitaxel treatment on H3K4me3 mark, d percentage of healthy, apoptotic and necrotic HeLa cells treated with compounds of the KDIPP series for 24 h in a dose-dependent manner, e percentage of healthy, apoptotic and necrotic HeLa cells treated with KDIPP15 (66 µM) or KDIPP51 (60 µM) for 24 h. Results are shown as mean ± SD from triplicates of two independent experiments