Fig. 5
From: Assessing histone demethylase inhibitors in cells: lessons learned
Apoptosis induced by different mechanisms is accompanied by methylation mark changes. a Images of HeLa cells treated with 100 nM paclitaxel for 24 h and stained with DAPI and a specific antibody for H3K4me3. The phase contrast image is shown to the left, blue DAPI nuclear stain in the middle and yellow H3K4me3 to the right. Arrows indicate apoptotic cells lacking the H3K4me3 mark, b number of HeLa cells treated with doxorubicin or paclitaxel in a dose-dependent manner based on counting of 12 fields, c immunofluorescence assay showing the effect of doxorubicin or paclitaxel treatment on H3K4me3 mark, d immunofluorescence assay showing the effect of doxorubicin or paclitaxel treatment on H3K27me3 mark, H3K9me2 mark and H3K36me2 mark, respectively