Fig. 3
From: Assessing histone demethylase inhibitors in cells: lessons learned
Effect of different KDM5 inhibitors on histone tail methylation status in HeLa cells. a Immunofluorescence assays of cells transfected with KDM5B and treated with the indicated inhibitors CPI-455 and KDOAM-32, respectively. Effect on H3K4me3 methylation is shown to be dose dependent in the upper panel. Number of cells in 20 fields, based on DAPI staining, is shown in the lower panel, b immunofluorescence assays of cells transfected with KDM5B and treated with inhibitors KDIPP15 or KDIPP51, as indicated. The dose-dependent effect on H3K4me3 methylation is shown in the upper panel. Number of cells in 20 fields, based on DAPI staining, is shown in the lower panel