Fig. 4

SMYD5 and trimethylated histone co-occupy genomic regions in ES cells. a Comparison of SMYD5-bioChIP and SMYD5-FLAG ChIP-Seq peaks. Scatter plot of log2 SMYD5 density at ChIP-enriched regions. b Heat map of SMYD5 ChIP-Seq densities. c Average profiles of SMYD5-bioChIP and SMYD5-FLAG density at SMYD5-FLAG enriched regions. d Western blot of H4K20me3, H4K20me2, and H4K20me1 in shLuc and shSmyd5 ES cells (top), and H4K20me3 in shLuc, shSmyd5, shLuc + WT, and shSmyd5 + WT (bottom). The bar graph (bottom right) shows H4K20me3 levels normalized to actin using ImageJ software (https://imagej.nih.gov/ij/). e Comparison of SMYD5 and H4K20me3 ChIP-Seq peaks. f Empirical cumulative distribution function (ECDF) for SMYD5-FLAG and SMYD5-bioChIP density at H4K20me3-enriched regions in ES cells