Fig. 9
From: Histone and DNA methylation control by H3 serine 10/threonine 11 phosphorylation in the mouse zygote
Effects of G9aFL-GFP and G9aCat-NLS-GFP expression in mouse zygotes on H3K9me2. a Shown are the representative images of PN4/5 stage zygotes stained with antibodies against H3K9me2. DNA is visualized by DAPI. m Maternal pronucleus, p paternal pronucleus, pb polar body. Scale bar 50 µm. b Quantification of H3Kme2 signals normalized against DNA signals in both parental genomes of zygotes at PN4/5. Relative signal intensities in control groups are set to 1. Statistical significance was calculated using t test (***P < 0.001)