Fig. 6
From: Histone and DNA methylation control by H3 serine 10/threonine 11 phosphorylation in the mouse zygote
Effects of H3.1/2/3-GFPS10A expression in mouse zygotes on 5mC and 5hmC. a Shown are the representative images of PN4/5 stage zygotes stained with antibodies against 5mC together with anti-ssDNA antibodies, or together with 5hmC antibodies. m Maternal pronucleus, p paternal pronucleus, pb polar body. Scale bar 50 µm. b Quantification of 5mC signals, normalized against ssDNA signals in both parental genomes of zygotes at PN4/5. Relative signal intensities in control groups are set to 1. Statistical significance was calculated using t test (***P < 0.001). c Quantification of 5hmC signals, normalized against DNA (PI propidium iodide) signals in both parental genomes of zygotes at PN4/5. Relative signal intensities in control groups are set to 1. Statistical significance was calculated using t test (***P ≤ 0.001)