Fig. 5

Examination by pyroassay of remaining known and putative imprinted gDMR. a Validation of pyrosequencing assays for remaining known imprinted gDMRs. Average methylation levels across all CpG in each assay were plotted. The median for all assays is indicated by a horizontal line, and these did not significantly differ from one another using Kruskal–Wallis (p value 0.1821). b Results for putative imprinted gDMR, plotted as in a. While differences between medians are not significant (Kruskal–Wallis p value 0.7291), a greater variance can be seen, particularly in heart. c Verification that pyrosequencing assays for both known and putative maternal gDMR showed low methylation in sperm, while the paternal assay returned high methylation levels. d Methylation is lost at all known and putative gDMR, though the decrease is very small at Dlk1-Gtl2 IG. Error bars represent s.e.m.; **p < 0.01; ***p < 0.001; n.s. not significant