Fig. 2

Mutation of Dnmt3a/b or Dnmt1 gives comparable methylation loss at imprinted loci. a Pyrosequencing displaying methylation loss at various imprinted loci in DNMT1-deficient cells (1KO) and cells lacking both DNMT3A and 3B (3abKO). The methylation levels drop significantly for 1KO and 3abKO for all gDMRs assayed. b Clonal methylation analysis of imprinted gDMRs (Snrpn and H19) in 1KO and 3abKO ESCs. c Summary of methylation levels from the clonal data in b. There was a significant decrease in methylation for H19 and Snrpn in all KO cell types compared to WT. Error bars indicate s.e.m. *p < 0.05; **p < 0.01; ***p < 0.001