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Fig. 5 | Epigenetics & Chromatin

Fig. 5

From: Systematic comparison of monoclonal versus polyclonal antibodies for mapping histone modifications by ChIP-seq

Fig. 5

Variability in H3K27ac patterns is dependent on the immunogen. a Scatter plots where each point represents the count of reads aligning to a non-overlapping, variably sized region as annotated in the chromatin regions determined by ENCODE mapping of the genome. Values are summed for the replicates of monoclonal and polyclonal H3K27ac antibodies. The red line (on the left and right plots) represents slope = 1. b H3K27ac antibodies in HeLa cells. R 2 is indicated for all points, TSS and enhancer regions

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