Fig. 3
From: Expression and epigenomic landscape of the sex chromosomes in mouse post-meiotic male germ cells
Post-meiotic Y chromosome is enriched in H3K27ac. Immunofluorescence detection of H3K27ac (red) in round spermatid nuclei. DAPI (blue) was used to stain nuclei. The most DAPI-dense round region is the chromocenter (i.e., the constitutive pericentromeric heterochromatin) the less DAPI-dense structure adjacent to the chromocenter is the post-meiotic sex chromatin (PMSC) and is indicated by an arrow. Anti-H3K9me3 (in green) marks the chromocenter and the PMSC in wild type (WT) round spermatids. Two types of staining were observed: either a brighter signal co-localizing with the PMSC (“WT PMSC+” panel), or a diffuse bright signal in the nucleus (“WT Diffuse” panel). As control, round spermatids with a large deletion of the Y chromosome (MSYq-) were used. See Additional file 4 for an extended panel