Fig. 7

RNA-seq analysis of gene expression changes in MYS-1-depleted XO worms. a X and autosome expression levels of genes with RPKM > 1 and expressed genes on individual chromosomes in XX and XO hermaphrodites. b Boxplot shows the distribution of log₂ expression ratios on the X, autosomes and all separate chromosomes between dpy-21(e428) mutant XX hermaphrodites and wild-type XX hermaphrodites. X chromosome was significantly derepressed compared to the autosomal average and all other chromosomes. Increased expression from the X was tested between X and autosome by one-sided Wilcoxon rank-sum test (***P < .001). c Boxplot shows the distribution of log₂ expression ratios on the X, autosomes and all separate chromosomes between XO hermaphrodites fed mys-1(RNAi) and XO hermaphrodites fed vector RNAi. X chromosome was statistically significantly repressed compared to the autosomal average and all other chromosomes, although the degree of repression was minor. Decreased expression from the X was tested between X and autosomes by one-sided Wilcoxon rank-sum test (*P < .05; **P < .01; ***P < .001). d The magnitude of log₂ expression ratios of X-linked (green) and autosomal genes (gray) between XO hermaphrodites fed mys-1(RNAi) and control XO plotted against dpy-21(e428) mutants and wild-type animals. The percentages of X-linked and autosomal genes with >10 % change in expression (±0.1 in log₂) in both knockdown and mutant compared to control worms are indicated in each quadrant. e Boxplots show the distribution of log₂ ratios on the X between dpy-21(e428) mutants and wild-type animals and mys-1(RNAi) XO hermaphrodites and control XO hermaphrodites. The first 3 sets of boxplots show the distribution of log₂ ratios of the top 5 %, top 10 % and top 15 % of highly differentially expressed X-linked genes in dpy-21(e428) mutants. The last set shows the distribution of all X-linked genes