Fig. 4

Hermaphrodite X chromosome compaction and male X chromosome decondensation occur simultaneously in development. Hermaphrodite and male embryos stained X paint FISH (red) to label X chromosome territories and DAPI (blue) to label DNA. a Plot of quantified percentages of nuclear volume occupied by X in wild-type hermaphrodite embryos (black) and male embryos (gray). Each point represents one embryo (n = 10 nuclei per embryo). Error bars indicate standard deviation. b Representative stained nuclei of wild-type hermaphrodites at various developmental stages (9 cell, 30 cell, 76 cell). Scale bars equal 5 µm. c Representative stained nuclei of male him-8(e1489) animals at various developmental stages (25 cell, 40 cell, 56 cell). Scale bars equal 1 µm