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Fig. 2 | Epigenetics & Chromatin

Fig. 2

From: An H4K16 histone acetyltransferase mediates decondensation of the X chromosome in C. elegans males

Fig. 2

The effects of MYS-1 on hermaphrodite X chromosomes. a Representative images of adult hermaphrodite intestinal nuclei in control, mys-1(RNAi) and htz-1(RNAi) stained with X paint FISH (red) to label X chromosome territories and DAPI (blue) to label DNA. Scale bars equal 5 µm. b Quantification of the percentage of nuclear volume occupied by X in control (n = 40), mys-1(RNAi; n = 36) and htz-1(RNAi; n = 37) hermaphrodites. Error bars indicate standard deviation. Asterisks indicate level of statistical significance by t-test analysis (***P < .001). c Representative images of adult hermaphrodite intestinal nuclei in control, mys-1(RNAi) and htz-1(RNAi) stained with chromosome I paint FISH (red) and DAPI (blue) to label DNA. Scale bars equal 5 µm. d Quantification of the percentage of nuclear volume occupied by chromosome I in control hermaphrodites (n = 20), mys-1(RNAi)(n = 20) and htz-1(RNAi; n = 20). Error bars indicate standard deviation. No statistically significant differences were found (P > 0.5). e Immunofluorescence with antibodies against DCC component DPY-27 (green), combined with X paint FISH (red), and DAPI (blue) staining. White arrowheads point to regions with strong DCC signal without strong X paint signal. f Unmated him-8 hermaphrodites subjected to control vector or mys-1(RNAi) were allowed to lay eggs. The percent of progeny that survived to adulthood is shown on the left, and the proportion of male and hermaphrodite worms among the surviving progeny is shown on the right. Asterisks indicate level of statistical significance by Chi-square test analysis (***P < .001)

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