Fig. 1

MYS-1 and putative Tip60-/NuA4-like complex members mediate X chromosome decondensation in males. a–c Adult intestinal nuclei stained with X paint FISH (red) to label X chromosome territories and DAPI (blue) to label DNA. a Representative stained nuclei of wild-type hermaphrodites, wild-type males and mys-1(n4075) males. Scale bars equal 5 µm. b Quantification of the percentage of nuclear volume occupied by X in hermaphrodites (number of nuclei (n) = 27), males (n = 27), mys-1(n4075) males (n = 20) and ssl-1(n4077) males (n = 20). c Quantification of the percentage of nuclear volume occupied by X in vector RNAi (n = 77), histone acetyltransferases (HATs) and histone acetyltransferases complex member (Tip60/NuA4, MOF-MSL, MOF-NSL) RNAi-treated males. mys-1(RNAi; n = 30), mys-2(RNAi; n = 24), mys-4(RNAi; n = 27), cbp-1(RNAi; n = 26), mrg-1(RNAi; n = 40), ssl-1(RNAi; n = 40), rha-1(RNAi; n = 40), wrd-5.1(RNAi; n = 40), and c16A11.4(RNAi; n = 40). Error bars indicate standard deviation. Asterisks indicate level of statistical significance by t-test analysis (***P < .001). d–f Adult intestinal nuclei stained with chromosome I paint FISH (red) to label chromosome I territories and DAPI (blue) to label DNA. d Representative stained nuclei of wild-type hermaphrodites, wild-type males and mys-1(n4075) males. Scale bars equal 5 µm. e Quantification of the percentage of nuclear volume occupied by chromosome I in hermaphrodites (n = 20), males (n = 17), mys-1(n4075) males (n = 18) and ssl-1(n4077) males (n = 16). f Quantification of the percentage of nuclear volume occupied by chromosome I in vector RNAi (n = 43), HATs, Tip60/NuA4, MOF-MSL and MOF-NSL member RNAi-treated males. mys-1(RNAi; n = 21), mys-2(RNAi; n = 23), mys-4(RNAi; n = 17), cbp-1(RNAi; n = 28), mrg-1(RNAi; n = 17), ssl-1(RNAi; n = 17), rha-1(RNAi; n = 20), wrd-5.1(RNAi; n = 17), and c16A11.4(RNAi; n = 20)