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Fig. 2 | Epigenetics & Chromatin

Fig. 2

From: High-throughput assessment of context-dependent effects of chromatin proteins

Fig. 2

Tethering of Gal4-HP1 results in global downregulation of integrated reporters. ac Reporter expression compared between TRIP pool transfected with Gal4-HP1 or controls Gal4 or HP1 only as quantified by NGS 2 days after transfection. df Quantification of reporters showing expression detectable by at least one sequencing read. g Correlation (Spearman) between expression in the Gal4-transfected control condition and downregulation upon HP1 tethering with linear regression (red) and standard error. Downregulation is quantified by ratio of expression in the Gal4-HP1-transfected condition over expression in the Gal4-transfected control condition; therefore, only reporters with detectable expression in both conditions are shown. h GFP reporter expression in a cell line with single integration induced with increasing levels of CuSO4 and transfected with Gal4-HP1 or Gal4 as quantified by qPCR. Amount of repression upon Gal4-HP1 tethering is constant over expression range. Data information: In (ac), a pseudocount of 0.1 was added to all expression values in order to be able to visualize non-expressed reporters on a log scale

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