Fig. 1
From: Imprinted DNA methylation reconstituted at a non-imprinted locus
Development of the Wnt1DR mutant allele. At the wild-type (WT) Rasgrf1 locus, the ICR includes the repeat region (large white triangles) directly adjacent to the DMD (D), and sites 1 and 2 (vertical black bars) from which secondary piRNAs are processed. The Wnt1DR targeting construct contains an FRT-flanked (small gray triangles) neomycin resistance cassette (Neo), loxP-flanked (small black triangle) repeats, the DMD, and a CGCG insertion in the Wnt1 3′ UTR (divot in UTR, underlined sequence is Wnt1DR specific) for allele-specific expression analysis. Primers for detecting Wnt1DR-specific methylation are denoted with black arrows. HindIII (H) and EcoRI (E) sites