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Table 2 Dynamic and structural parameters of histone-FP-labeled chromatin domains obtained with FCS at different nuclear localizations

From: Dynamic properties of independent chromatin domains measured by correlation spectroscopy in living cells

   n Loop-rosette, blob; theta-solvent conditionsa Loop-rosette; good-solvent conditionsb Globularb
τ 1 (ms) R g (nm) gc (Mb) τ 1 (ms) R g (nm) gc (Mb) R g (nm) gc (Mb)
H1-EGFP
untreated
Perinuclear 35 91 ± 6 245 ± 5 0.80–1.12 100 ± 6 289 ± 6 1.31–1.83 240 ± 5 0.75–1.05
Perinucleolar 34 78 ± 6 234 ± 6 0.70–0.98 94 ± 5 283 ± 5 1.23–1.73 235 ± 4 0.71–0.99
Euchromatin 62 161 ± 15 297 ± 9 0.83–1.16 191 ± 20 359 ± 12 1.47–2.05 298 ± 10 0.84–1.17
H2A-EGFP
untreated
Perinucle(ol)ar 84 83 ± 7 238 ± 4 0.73–1.03 90 ± 8 279 ± 4 1.18–1.65 232 ± 4 0.68–0.95
Euchromatin 84 165 ± 11 299 ± 9 0.85–1.19 188 ± 14 356 ± 13 1.43–2.00 297 ± 11 0.83–1.16
H2B-mCherry
untreated
Perinucle(ol)ar 84 94 ± 6 249 ± 4 0.84–1.18 102 ± 7 291 ± 4 1.34–1.88 242 ± 3 0.77–1.08
Euchromatin 84 174 ± 10 304 ± 9 0.89–1.25 195 ± 12 361 ± 12 1.49–2.09 300 ± 10 0.86–1.12
H1-EGFP
TSA-treated
Perinucle(ol)ar 25 292 ± 34 362 ± 14 1.65–2.31 366 ± 49 445 ± 20 3.07–4.30 370 ± 17 1.77–2.47
Nucleoplasm 18 307 ± 37 368 ± 15 1.74–2.43 384 ± 51 453 ± 20 3.24–4.54 377 ± 17 1.87–2.61
H1-EGFP
ATP-depleted
Perinucle(ol)ar 17 303 ± 51 367 ± 21 1.72–2.41 388 ± 74 454 ± 29 3.26–4.57 378 ± 24 1.88–2.64
Nucleoplasm 25 278 ± 43 356 ± 18 1.57–2.20 351 ± 59 439 ± 25 2.95–4.13 365 ± 21 1.69–2.37
  1. (mean value ± standard error; min. value–max. value)
  2. τ 1—decay time of the first polymer relaxation mode, R g —radius of gyration of topologically and dynamically independent chromatin domain, gc—genomic content of topologically and dynamically independent chromatin domain
  3. aRelaxation times and radii of gyration are numerically identical for loop-rosette conformation under theta-solvent conditions and for blob conformation
  4. bRelaxation times are numerically identical for loop-rosette conformation under good-solvent conditions and for globular conformation