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Fig. 5 | Epigenetics & Chromatin

Fig. 5

From: Fine-tuning of chromatin composition and Polycomb recruitment by two Mi2 homologues during C. elegans early embryonic development

Fig. 5

LET-418 and CHD-3 modulate histone marks and MES-2 recruitment at target genes. a ChIP-Seq validation of LET-418 binding patterns at four selected target genes by ChIP-qPCR in a wild-type genetic background. LET-418 ChIP-Seq profile (IGV browser—red) is represented at the top of the panel for the ric-3, xol-1, asic-2, and ins-39 target genes (blue boxes). CHD-3::StrepIII::HA and MES-2::EGFP::3xFLAG enrichments were measured by ChIP-qPCR at these loci. Results are represented as % of the total DNA input added in each IP. Error bars qPCR measurement standard error. b ChIP-qPCR of H3K4me3, H3K27me3, and MES-2::EGFP::3xFLAG enrichment at target genes promoter and gene body, and mRNA expression levels of the same target genes, in the indicated genotypes. Results are represented as fold induction of the DNA recovery versus control (RNAi)-treated wild-type embryos, and for histone modifications only were normalized to total H3 levels, to obtain the histone mark enrichment independently of nucleosome concentration. mRNA levels of asic-2, xol-1, ins-39, and ric-3 measured by qRT-PCR, represented as fold induction of mRNA expression versus control(RNAi)-treated wild-type embryos, and normalized to four housekeeping mRNA levels using the Best Keeper method. All ChIP-qPCR and qRT-PCR experiments were performed at least in duplicate, and one representative experiment is displayed here

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