Fig. 3

Genomic element enrichment of genes with acquired bivalency under hypoxia. a Both H3K4me3 enrichment and H3K27me3 enrichment increase in genes in response to hypoxia, with a decrease upon reoxygenation which is most pronounced for the H3K27me3 mark. The total AUC (log2-transformed) of all ChIP-seq peaks in a gene is represented on the y axis (mean over all genes and standard error are shown). Star symbol indicates a significant difference compared to t = 0 (i.e., normoxia; p < 0.05, Wilcoxon signed-rank test). b Distribution of H3K4me3- and H3K27me3-enriched promoters with (top) high CpG, (middle) intermediate and (bottom) low CpG-content. c Overlap of bivalent genes in this study with bivalently marked genes in embryonal stem cells (ES cells [27]), transcription factor (TF [25]; in gray) genes, genes containing promoter CpG islands (CpG [25]; in white) and genes whose TSS coincides with transposon exclusion zones (TEZ [25]; in black)