Fig. 2From: Quantitative analysis of ChIP-seq data uncovers dynamic and sustained H3K4me3 and H3K27me3 modulation in cancer cells under hypoxiaValidation of sustained and dynamic H3K4me3 marking. Bioinformatic calling of enriched regions (gene tracks shown at left side for each locus) was validated by ChIP-PCR (bar plots; shown at right side for each locus) for a number of representative epigenetic profiles. Data for all time points are shown: normoxia (0), hypoxia 8 h (8), hypoxia 24 h (24) and reoxygenation (+8). CCNA2, DPM1 and NOL11 for genes that show sustained H3K4me3 marking in relation to changes in oxygen pressure (left panels); ATP2A3, FOXF1 and IGFBP4 for genes that show dynamic modulation of H3K4me3 marking in relation to changes in oxygen pressure (right panels); gene-specific ChIP-PCR analyses are part of a biological study published elsewhere [10]. Diamond symbol indicates direction of transcription, black triangle indicates approximate position of primer set used for validation, and star symbol indicates a significant difference compared to t = 0 (i.e., normoxia; p < 0.05, Wilcoxon signed-rank test)Back to article page