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Fig. 2 | Epigenetics & Chromatin

Fig. 2

From: Quantitative analysis of ChIP-seq data uncovers dynamic and sustained H3K4me3 and H3K27me3 modulation in cancer cells under hypoxia

Fig. 2

Validation of sustained and dynamic H3K4me3 marking. Bioinformatic calling of enriched regions (gene tracks shown at left side for each locus) was validated by ChIP-PCR (bar plots; shown at right side for each locus) for a number of representative epigenetic profiles. Data for all time points are shown: normoxia (0), hypoxia 8 h (8), hypoxia 24 h (24) and reoxygenation (+8). CCNA2, DPM1 and NOL11 for genes that show sustained H3K4me3 marking in relation to changes in oxygen pressure (left panels); ATP2A3, FOXF1 and IGFBP4 for genes that show dynamic modulation of H3K4me3 marking in relation to changes in oxygen pressure (right panels); gene-specific ChIP-PCR analyses are part of a biological study published elsewhere [10]. Diamond symbol indicates direction of transcription, black triangle indicates approximate position of primer set used for validation, and star symbol indicates a significant difference compared to t = 0 (i.e., normoxia; p < 0.05, Wilcoxon signed-rank test)

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