Fig. 2

Identification of individual protein components in Pax6-containing immunoprecipitates. a Subunit structure of Mll1/2 and Set1a complexes and number of specific peptides (n) of these subunits identified by LC–MS/MS. b Pax6-immunoprecipitates contain additional subunits of multiple chromatin-modifying and remodeling complexes, including BAF, ACF, RCF, WICH, NuRD, NuA4, HAT, and HMT. The catalytical subunits of these complexes are shown in green. c Co-IP validation of Pax6 in immunoprecipitates obtained using Mll1, Mll2, Set1a, Wdr5, Rbbp5, Cfp1, Ash2l, and Dpy30 antibodies. d Co-IP validation of the FACT complex subunits Ssrp and Spt16 in Pax6-immunoprecipitates. e Co-IP validation of the Snf2h (Smarca5) in Pax6-immunoprecipitates. IgG-immunoprecipitates were used as control. Protein markers are shown in kDa