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Fig. 1 | Epigenetics & Chromatin

Fig. 1

From: Pax6 associates with H3K4-specific histone methyltransferases Mll1, Mll2, and Set1a and regulates H3K4 methylation at promoters and enhancers

Fig. 1

Pax6-immunoprecipitates contain histone methyltransferase activities specific for recombinant histone H3. a In vitro HMT assay using recombinant histone octamers. The Pax6-immunoprecipitates were used at 1x (+) and 2x (++) amounts. Input represents the lens cell nuclear extract. IgG-immunoprecipitates were used as a control. b In vitro HMT assay using recombinant H2A and H3 histones. IgG-immunoprecipitates were used as a control. c In vitro HMT assay using unmodified, mono-, di- and trimethylated H3K4 peptides (residues 1–20) in the presence of Pax6-immunoprecipitates. d In vitro HMT assay using unmodified, mono-, di- and trimethylated H3K4 peptides in the presence of Wdr5-immunoprecipitates. The HMT activities of control IgG-immunoprecipitates were subtracted in both c, d. (error bars = ±s.d.)

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