Skip to main content
Fig. 5 | Epigenetics & Chromatin

Fig. 5

From: The cancer-associated CTCFL/BORIS protein targets multiple classes of genomic repeats, with a distinct binding and functional preference for humanoid-specific SVA transposable elements

Fig. 5

BORIS binding to SVA elements in vitro is based on binding to sequences in the VNTR. a The design and positions of EMSA probes based on a test-case SVA-D element (hg19, chr11:107782497–107784211). The two CTCF-binding motif-matching sequences are shown in probe 5 (MEME p value 7.95e−06) and probe 6 (p value 1.32e−05). Red bars denote two unique sequences acquired by this retrotransposon. b EMSA shows that the tested SVA-D element contains at least two distinct BORIS and CTCF binding sites in the VNTR region, with binding sensitive to 5metC methylation in vitro. The binding in probe 3 was shown to be due to the presence of a unique sequence

Back to article page