Fig. 4

SVA repeats are preferentially bound by BORIS with a strong preference for VNTR region. a The chart showing the dominance of SVA elements among the repeats from Additional file 1: Table S2 that are strongly bound by BORIS (×4 enrichment or more by ChIP-chip) with no enrichment for CTCF (×1 or less). b BORIS occupancy is associated with SVAs repeats in K562 cells. The heatmap demonstrates the ChIP-seq enrichment of BORIS occupancy at SVAs element in K562 cells compared to input. The tag density was subjected to k-means ranked clustering with four clusters expected. c The ChIP-seq tag density distribution for a “canonical” full-length SVA-D element from Repbase shows that BORIS is clustered at the center of the element in a pattern complementary to CTCF. The normalized counts were binned along the DNA sequence (histogram bars) with the fit line applied accordingly. d The schematic structure of SVA-D element in e. e A diagonal alignment plot of DNA sequence for SVA-D in c, d indicating that BORIS enrichment corresponds to the VNTR region