Fig. 5

TORC1 and histone H3 prevent cell death through vacuole dysfunction and altered pH homeostasis. a H3WT and H3K37A transformed with control vector or the indicated galactose-inducible expression vectors were spotted to the indicated plates. b H3WT and H3K37A were cultured to log phase and then mock or 20 nM rapamycin treated for 30′ before staining with FM-464 (red, stains vacuole membrane) and 5(6)-CFDA (green upon acidification). Arrows indicate cells where significant fluorescence is detectable. c Experiment was performed as in b, and cells were sampled, stained, and analyzed by flow cytometry at the indicated times post-rapamycin treatment. Data are the average and SD of 3 independent experiments. d H3WT, H3K37A, and ρ° derivatives in these backgrounds were spotted to control synthetic complete (SC) plates or 20 nM rapamycin SC plates that were either non-buffered or buffered to pH 6.0 with MES