Fig. 3

TORC1 and histone H3 regulate Nhp6a nuclear localization independently of the FACT histone chaperone complex. a Mock or 1-h 20 nM rapamycin-treated H3WT, H3K37A, and H3K37R cells expressing Nhp6a-EGFP were analyzed by confocal microscopy. Cell outlines are indicated by the line trace. b Quantification of Nhp6a nuclear localization from three independent experiments with the average and standard deviation (SD) plotted. One-way ANOVA was performed across all categories which is indicated by the dashed line, while the solid black line indicates the pairwise comparison analyzed by Student’s t test. *P < 0.05; **P < 0.01. c. As in a, except cells expressing Spt16-EGFP were analyzed. Brightfield images for a and c are in Additional File 1: Figure S3