Fig. 2

Histone H3 and TORC1 differentially regulate Nhp6a and Ixr1 cellular localization. Confocal microscopy and brightfield images of H3WT and H3K37A expressing either Nhp6a-EGFP (a, b) or Ixr1-EGFP (c, d). Cells were mock or 20 nM rapamycin treated for 1 h. The outline of individual cells is demarcated by the line trace. The nucleus is indicated by Hoechst (blue) staining. Scale bar indicates 5 μm for all live cell confocal images