Fig. 7

Proposed model for GAF-mediated transcriptional pausing. Genes with high SS-dinucleotide contents around and upstream of the TSS are occupied by much higher levels of upstream nucleosomes (dark circle) than others. This organization prevents the loading and assembly of the transcriptional complex. The occupancy of GAF results in the recruitment and cooperative interactions with NURF, NELF and BAB1 to facilitate local nucleosome remodeling and provide accessible space for the assembly of the transcriptional complex and subsequently triggers its transition to the paused state