Absence of genomic hypomethylation or regulation of cytosine-modifying enzymes with aging in male and female mice

Table 1 Meta-analysis of age-related DNMT and TET expression in brain

Entrez ID	Symbol	Chr	Name	Coeff.	SE	t	p value
80312	TET1	10	Tet methylcytosine dioxygenase 1	−0.00192	0.006	−0.34	0.73
54790	TET2	4	Tet methylcytosine dioxygenase 2	−0.00002	0.005	−0.004	1.0
200424	TET3	2	Tet methylcytosine dioxygenase 3	−0.00033	0.005	−0.064	0.95
1786	DNMT1	19	DNA (cytosine-5-)-methyltransferase 1	0.00784	0.007	1.093	0.27
1788	DNMT3a	2	DNA (cytosine-5-)-methyltransferase 3 alpha	0.004	0.004	0.980	0.33
1789	DNMT3b	20	DNA (cytosine-5-)-methyltransferase 1	−0.00009	0.004	-0.02	0.98

To test whether the expression levels of these genes change with age, we used a linear mixed-effects (lme4) model: [GeneExpression ~ Age: TissueID + (1|ExperimentID) + (1|PlatformID)]. Shown above is the age–tissue interaction term for brain. “Coef” describes the estimated coefficient of the change in Z-score per year for the expression of each gene, the standard error (SE), and t-statistic for the analysis. The p value shows that none of the estimated gene expression changes with age are statistically significant

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