Fig. 2
Gene expression analysis of DNMTs. mRNA levels of DNMTs were assessed by qPCR in hippocampi of both sets of mice. Data were analyzed both with sexes combined (one-way ANOVA on age) and with sexes analyzed independently (two-way ANOVA on age and sex). No consistent changes were evident in a DNMT1, b pan-DNMT3a (combination of long, a1, and short, a2, isoforms), c DNMT3a1, or e DNMT3b. d DNMT3a2 was not detectable. Data are scaled to zero for the young group in the combined sexes analysis and to young female for the sex separated analysis with the y-axis scale presented as % change from zero. Boxes encompass the 25th to 75th percentile with bars indicating 10th and 90th percentiles. Median lines are indicated in the boxes