Fig. 4

Heatmap of histone modification signals at 114 differentially methylated regions in monocyte (Mo) versus macrophage (Mac). The heatmap shows the log2 ratio of ChIP signal over input at the DMRs plus 1 kb flanking regions for six different histone modifications. k-means clustering revealed three cluster with distinct chromatin signatures. Cluster 1 comprises DMRs that gain in macrophages the signature of active enhancers in non-transcribed regions, cluster 2 comprises DMRs that gain in macrophages the signature of active enhancers in actively transcribed regions and cluster 3 comprises DMRs with the signature of promoters or enhancers that are active in both monocyte and macrophage. Clusters and histone marks with significant change during ​monocyte-to-macrophage differentiation (p < 0.001, paired Student’s t test with Bonferroni correction) are highlighted with a bold light blue border